Induction of mitotic arrest in human CRC cells and inhibition of Biology Diagrams In this cell line, the ablation of the 14-3-3-ฯ locus also abolishes the G2 arrest and causes mitotic catastrophe linked to the premature entry of cyclin B/Cdk1 into the nucleus (Chan et al., 1999).
Induced cell cycle arrest is the use of a chemical or genetic manipulation to artificially halt progression through the cell cycle.Cellular processes like genome duplication and cell division stop. [1] It can be temporary or permanent. [1] It is an artificial activation of naturally occurring cell cycle checkpoints, induced by exogenous stimuli controlled by an experimenter. In turn p21 can cause G1 arrest, whereas Bax can cause apoptosis II. Also, I discuss that mitotic depletion of short-lived proteins collaborates with mitotic phosphorylation of p53, Bcl-2 and BclxL. Finally this article clarifies notions of apoptosis-prone and -reluctant cells and mitotic catastrophe.
A link between mitotic defects and mitotic catastrophe: detection and ... Biology Diagrams
Suppression of mitotic telomere damage by enhanced expression of TRF2, or the inhibition of either caspase-3/7 or DNA-PK during mitotic arrest, promotes subsequent cell survival. Microtubule inhibitors such as paclitaxel, vincristine and nocodazole cause mitotic arrest. Cell fate determination during mitotic arrest may seem like a puzzle. If a cell cannot exit mitosis, 1it undergoes apoptosis.-4 On the other hand, apoptosis may require mitotic 2,5,6exit. Apoptosis is often p53-independent but can be p53-dependent.1,6-12 Prolonged mitotic arrest with the same drugs causes apoptosis during mitotic arrest in apoptosis-sensitive cell lines; in less sensitive lines, cells undergo slippage without division into tetraploid G1, which may be followed by p53-dependent arrest, apoptosis, or another round of mitosis (Rieder and Maiato, 2004; Gascoigne and Taylor, 2008
Mitotic arrest is defined as the difference between mitotic exit and mitotic entry. Central panel: barplots with the percentages of the different classes of Clb2 behavior in wild-type or GAL1-Mad2 cells arrested indefinitely in nocodazole. For the definition of the cell classes see S2A Fig. Right panel: cumulative distribution of APC/C For example, increased expression of mitotic arrest deficient 2 (Mad2)-interacting protein p31 comet causes cellular senescence, which is accompanied by mitotic catastrophe . Such combination of drugs causes prolonged arrest of the cell cycle in the G2/M phase, accumulation of micronuclei, and chromosomal aberrations indicative of mitotic Mitotic arrest induced by antimitotic drugs can cause apoptosis or p53-dependent cell cycle arrest. It can also cause DNA damage, but the relationship between these events has been unclear. Live, single-cell imaging in human cancer cells responding to an antimitotic kinesin-5 inhibitor and additiona โฆ
